e2 ubch5α r d systems e2 616 Search Results


e2 ubch5α r d systems e2 616  (R&D Systems)
95
R&D Systems e2 ubch5α r d systems e2 616
E2 Ubch5α R D Systems E2 616, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/e2+ubch5%CE%B1+r+d+systems+e2+616/Recombinant+Human+UbcH5a%2FUBE2D1+Protein%2C+CF/pmc06062852-725-0-1
Average 95 stars, based on 1 article reviews
e2 ubch5α r d systems e2 616 - by Bioz Stars, 2026-09
95/100 stars
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recombinant human ubiquitin protein, cf  (Bio-Techne corporation)
96
Bio-Techne corporation recombinant human ubiquitin protein, cf
Recombinant Human Ubiquitin Protein, Cf, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/e2+ubch5%CE%B1+r+d+systems+e2+616/Recombinant+Human+Ubiquitin+Protein%2C+CF/custom%40u-100h%4030040244
Average 96 stars, based on 1 article reviews
recombinant human ubiquitin protein, cf - by Bioz Stars, 2026-09
96/100 stars
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recombinant human ubiquitin activating enzyme (ube1), cf  (Bio-Techne corporation)
95
Bio-Techne corporation recombinant human ubiquitin activating enzyme (ube1), cf
Recombinant Human Ubiquitin Activating Enzyme (Ube1), Cf, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/e2+ubch5%CE%B1+r+d+systems+e2+616/Recombinant+Human+Ubiquitin+Activating+Enzyme+(UBE1)%2C+CF/custom%40e-305%4030040244
Average 95 stars, based on 1 article reviews
recombinant human ubiquitin activating enzyme (ube1), cf - by Bioz Stars, 2026-09
95/100 stars
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hrp conjugate  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology hrp conjugate
Hrp Conjugate, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/e2+ubch5%CE%B1+r+d+systems+e2+616/goat+anti-mouse+IgG-HRP/pmc06062852-524-80-82
Average 96 stars, based on 1 article reviews
hrp conjugate - by Bioz Stars, 2026-09
96/100 stars
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materials atp e1  (R&D Systems)
95
R&D Systems materials atp e1
Set-up for component-based cell-free ubiquitination assay
Materials Atp E1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/e2+ubch5%CE%B1+r+d+systems+e2+616/Recombinant+Human+Ubiquitin+Activating+Enzyme+(UBE1)%2C+CF/pmc06062852-524-0-4
Average 95 stars, based on 1 article reviews
materials atp e1 - by Bioz Stars, 2026-09
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Image Search Results


Set-up for component-based cell-free ubiquitination assay

Journal: Current protocols in chemical biology

Article Title: Design and functional characterization of synthetic E3 ubiquitin ligases for targeted protein depletion

doi: 10.1002/cpch.37

Figure Lengend Snippet: Set-up for component-based cell-free ubiquitination assay

Article Snippet: Materials ATP E1 (Ube1; R&D Systems E-305) E2 (UbcH5α;R&D Systems E2-616) Ubiquitin (R&D Systems U-100H) Purified uAb, target, and control proteins (see Basic Protocol 2 ) Ubiquitination reaction buffer, 10× (see recipe) Laemelli buffer, 2× (see recipe) SDS-PAGE running buffer, 1× (see recipe) SDS-PAGE gel (4–20%) Polyvinylidene difluoride (PVDF) membrane Powdered milk Tris-buffered saline with Tween 20 (TBST; see recipe) Anti-6×His tag antibody, HRP conjugate (abcam ab1187) Anti-ubiquitin antibody, mouse (MilliporeSigma 05-944) Antibody specific for target protein Goat anti-mouse antibody, HRP conjugate (Santa Cruz sc-2005) HRP conjugated secondary antibody for detection of anti-target antibody Chemiluminescent Substrate Equipment Power supply Gel electrophoresis system Gel transfer system Gel imaging system capable of chemiluminescence detection Protocol Time Course Assay list-behavior=simple prefix-word= mark-type=none max-label-size=2 1a Calculate reaction volume based on number of time points desired (10 µL/time point).

Techniques:

Journal: Current protocols in chemical biology

Article Title: Design and functional characterization of synthetic E3 ubiquitin ligases for targeted protein depletion

doi: 10.1002/cpch.37

Figure Lengend Snippet:

Article Snippet: Materials ATP E1 (Ube1; R&D Systems E-305) E2 (UbcH5α;R&D Systems E2-616) Ubiquitin (R&D Systems U-100H) Purified uAb, target, and control proteins (see Basic Protocol 2 ) Ubiquitination reaction buffer, 10× (see recipe) Laemelli buffer, 2× (see recipe) SDS-PAGE running buffer, 1× (see recipe) SDS-PAGE gel (4–20%) Polyvinylidene difluoride (PVDF) membrane Powdered milk Tris-buffered saline with Tween 20 (TBST; see recipe) Anti-6×His tag antibody, HRP conjugate (abcam ab1187) Anti-ubiquitin antibody, mouse (MilliporeSigma 05-944) Antibody specific for target protein Goat anti-mouse antibody, HRP conjugate (Santa Cruz sc-2005) HRP conjugated secondary antibody for detection of anti-target antibody Chemiluminescent Substrate Equipment Power supply Gel electrophoresis system Gel transfer system Gel imaging system capable of chemiluminescence detection Protocol Time Course Assay list-behavior=simple prefix-word= mark-type=none max-label-size=2 1a Calculate reaction volume based on number of time points desired (10 µL/time point).

Techniques: Concentration Assay